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Tissue distribution of hAps1, hAps2 and β-actin. PCR using human tissue first-strand cDNA as template was performed with primers specific for β-actin and for hAps1 or hAps2. 35 cycles of amplification were used for β-actin, 40 cycles for hAsp1 and hAps2.

Journal: BMC Biochemistry

Article Title: Cloning and characterisation of hAps1 and hAps2, human diadenosine polyphosphate-metabolising Nudix hydrolases

doi: 10.1186/1471-2091-3-20

Figure Lengend Snippet: Tissue distribution of hAps1, hAps2 and β-actin. PCR using human tissue first-strand cDNA as template was performed with primers specific for β-actin and for hAps1 or hAps2. 35 cycles of amplification were used for β-actin, 40 cycles for hAsp1 and hAps2.

Article Snippet: The tissue distribution of hAps1 and hAps2 expression was determined by PCR from human tissue cDNA samples (human cDNA panels, Clontech) and human cDNA libraries (testis from Clontech, brain from Dr Peter Cheung of the MRC Protein Phosphorylation Unit, Wellcome Trust Biocentre, Dundee, Scotland, UK). β-Actin-specific and hAps1 and hAps2-specific primers were used.

Techniques: Amplification